A Win–Loss Interaction on Fe0 Between Methanogens and Acetogens From a Climate Lake

Diverse physiological groups congregate into environmental corrosive biofilms, yet the interspecies interactions between these are seldom examined. We, therefore, explored Fe 0 -dependent cross-group acetogens and methanogens from lake sediments. On , were more metabolically active when decoupled methanogens, whereas coupled with acetogens. This suggests an opportunistic (win–loss) interaction on (loss) (win). Clostridia Methanobacterium major candidates doing acetogenesis methanogenesis after four transfers (metagenome sequencing) only detected 11 (amplicon . Since abiotic H 2 failed to explain high metabolic rates we examined whether cell exudates (spent media filtrate) promoted -evolving reaction above controls. Undeniably, spent filtrate generated three- four-fold than controls, which could be partly explained by thermolabile enzymes non-thermolabile constituents released cells. Next, metagenome for candidate enzymes/shuttles that catalyze evolution found hydrogenases almost complete pathway flavin biosynthesis in Clostridium. Clostridial ferredoxin-dependent [FeFe]-hydrogenases may catalyzing explaining significant evolved exposed It is typical of secrete other small molecules lytic purposes. Here, they such enhance their own electron uptake extracellular donors but indirectly make -consuming neighbors— —fare five times better presence. The particular promoting remain determined. However, postulate a static environment like crust biofilms sediments, less extend corrosion long ceased, exploiting secreted